CRISPR-Cas9 Genome Editing

How are components of prokaryotic adaptive immunity used to edit genomes?

Abstract

CRISPR-Cas9 genome editing is a POGIL learning cycle activity designed for introductory biology students at the college or university level. Genome editing is a technique that allows scientists to direct changes in the DNA of an organism. Here, students will explore how the prokaryotic adaptive immune response is modified to conduct CRISPR-Cas9 genome editing. At the end of this activity, students will be able to describe the types of genome edits that can be made using CRISPR-Cas9 technology and predict how these edits impact gene function. This is important for undergraduate students interested in careers in medicine, allied health care professions, and biological research as CRISPR-Cas9 genome editing is used for a broad range of applications, including making robust agricultural products and designing therapies for heritable disease.

Level: Undergraduate
Setting: Classroom 
Activity Type: Learning Cycle,
Discipline: Biology
Course: Introductory biology
Keywords: Genome editing